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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Topic 1: Hematology20-25%- Red blood cell physiology and disorders
- Routine and automated hematology procedures
- White blood cell morphology and diseases
- Hemostasis and coagulation testing
Topic 2: Clinical Chemistry20-25%- Electrolytes, acid-base balance, and blood gases
- Carbohydrates, lipids, proteins, and enzymes
- Toxicology and liver/kidney function tests
- Endocrinology and therapeutic drug monitoring
Topic 3: Microbiology15-20%- Identification of pathogenic microorganisms
- Bacteriology and antimicrobial susceptibility
- Specimen collection and culture techniques
- Mycology, parasitology, and virology
Topic 4: Blood Banking (Immunohematology)15-20%- Antibody detection and identification
- Blood group systems and antigens
- Blood components and storage
- Transfusion reactions and adverse events
- Compatibility testing
Topic 5: Immunology and Serology5-10%- Autoimmune and immunodeficiency disorders
- Infectious disease serology
- Immunologic testing methodologies
- Immune system principles and components
Topic 6: Urinalysis and Other Body Fluids5-10%- Semen and amniotic fluid testing
- Physical, chemical, and microscopic analysis
- Cerebrospinal, serous, and synovial fluids
- Urine formation and composition
Topic 7: Laboratory Operations5-10%- Quality control and quality assurance
- Laboratory mathematics and instrumentation
- Pre-analytical, analytical, and post-analytical processes
- Laboratory safety and regulatory compliance

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Kell, Kidd, and Duffy are usually warm reacting IgG antibodies. The most common P system antibody is anti-P1, which is a frequently naturally occurring low titer IgM antibody, seen in most P2 individuals.
Blood bank
Which of the following blood groups is most frequently associated with cold agglutinins:

A) Duffy
B) P
C) Kell
D) Kidd


2. Certain recipients have increased risk for developing TA-GVHD. They are:
Neonates less than 4 months of age
Fetuses
Recipients with a congenital or acquired immunodeficiency, such as bone marrow or stem cell recipients, and patients receiving chemotherapy recipients of donor units from a blood relative Which of the following patients are at risk for transfusion-associated graft versus host disease (TA-GVHD) and require irradiated cellular blood products? (Choose all that apply)

A) Patient receiving chemotherapy who are immunocompromised.
B) Patients with chronic anemias.
C) Recipients of donor units known to be from a blood relative.
D) Patients with a history of allergic reactions.
E) Neonates less than 4 months of age


3. Though automated extraction machines have many benefits over manual methods the costs are high and generally require a high throughput of samples in order to justify the costs.
Automated extraction has many benefits over the traditional manual methods. The most important benefit is that the nucleic acid isolated is constantly consistent. There is a reduced amount of manipulation with dramatically decreases the chance of cross contamination. Also, automated extraction machines are considered moderate complexity and can be performed by a wider variety of laboratory professionals.
All of the following are considered benefits of automated isolation and extraction equipment EXCEPT:

A) Automated isolation equipment provides consistent results
B) Automated isolation equipment is considered moderate complexity
C) Automated isolation equipment is cheaper and more practical
D) Automated isolation equipment requires less manipulation


4. Cholesterol levels do not fluctuate as triglycerides, fatty acids, lipoproteins, and chylomicrons do after an individual eats a meal. This is the reason that patients are told to fast; the laboratory needs a fasting sample to determine a lipid profile or panel which includes the latter four components.
Which of the following lipid tests is LEAST affected by the fasting status of the patient?

A) Triglycerides
B) Lipoproteins
C) Chylomicrons
D) Cholesterol
E) Fatty acids


5. Skeletal deformations result from the increased erythropoiesis that occurs in beta thalassemia major. Children with beta thalassemia intermedia may demonstrate some facial bone deformity, however this is not common.
Beta thalassemia minor rarely causes any physical signs or symptoms and beta thalassemia minima is completely asymptomatic.
Skeletal deformations are most commonly present in which of the following beta thalassemias?

A) Beta thalassemia intermedia
B) All of the beta thalassemias equally
C) Beta thalassemia minor
D) Beta thalassemia major
E) None of the beta thalassemias


Solutions:

Question # 1
Answer: B
Question # 2
Answer: A,C,E
Question # 3
Answer: C
Question # 4
Answer: D
Question # 5
Answer: D

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